I have worked with many applications (mostly large and not Access) that store calculated values. Accounting applications do it and it works fine because you "close" accounting periods. Once the period is closed, you can no longer modify or add transactions for it. At that point, you may as well store the period totals. It is also done in inventory applications under similar conditions. Periods are closed and on-hand quantities are stored. With inventory applications they also do periodic stock takes. Those records are stored and used as the starting point for running balances. So instead of going back to May 1st, 1956 to calculate the on hand quantity of paper cups, you find the most recent stock take and sum from there.
There is a theme though. Balances are only stored when updates to previous periods are disallowed. If that rule weren't enforced, every transaction would result in recalculating every intervening balance.
In all cases, the storing of calculated balances in a transactional system is a violation of normal forms. Yet, knowledgable designers do it for practical reasons in systems with high transaction volumes but only in strictly limited situations.
So the answer to your question is - not likely. The day's transactions would be summed after the close of business (period closed) at which point they could be saved.