Ken's answer is right but doesn't tell you the why of the why.
An autonumber is a special case of a LONG in Access. It is generated by touching the recordset of the underlying table with an AddNew function (whether YOU do it in VBA directly on the recordset or a form or query does it behind the scenes.) If you abort the operation before you do the Update, it is too late for the thing in the TableDef that tracks the next autonumber.
Therefore, autonumbers are not continuously numbered. They can have gaps. They are, essentially, unpredictable.
When you ask to put a letter before, after, or in the middle of an autonumber sequence, it implies that the autonumber has some other meaning than just a record identifier. Even if the letter is constant, the fact that you think you need it means you have a use for it somewhere.
When that is the case, you usually want predictability for the number. And therein lies your difficulty. Autonumbers are inherently not predictable. Now, here is where you have a couple of choices, neither of which is necessarily nice.
First, if the letter is a constant (i.e. ALWAYS 'E') then why do you care? Adjust your attitude.
Second, if the letter is NOT a constant then you cannot use autonumbering to define this item at all. Because an autonumber MUST be independent of any and all other fields in the record. More technically, the other fields of the record are identified by the autonumber, not the other way around. This is a normalization thing, when examined at its most basic level. Fields in a record either depend on their prime keys or shouldn't be in the record. THAT rule...
In the second case, you need to consider a way to determine something like the maximum number assigned among all records with the same letter. Then you have to recognize that numbers and letters are interpreted differently, so having a key based on letters and numbers means at least a two-part compound primary key. Legal - but trickier to manage because of issues in destructive interference. Like, if two users attempt to add a record with the same letter key at the same time, only one of them will correctly add the record. The other adds a duplicate.
Search this forum for non-autonumber keys.